Difference between revisions of "Part:BBa K1699005"

Revision as of 20:00, 5 September 2015

gRNA for dCas9-VP64 targeting synthetic activation promoter pMLPm under U6 promoter

gRNA for dCas9-VP64 targeting synthetic activation promoter pMLPm under U6(RNA pol III) promoter.

Usage and Biology

gRNA is part of CRISPR/Cas9 complex. guide RNA is a hundred base-long molecule with a unique two dimensional structure which binds Cas9 and guides it to a dsDNA sequence complementary to 21-22 base pairs on the 5' end of the molecule. gRNA scaffold sequence for SaCas9 was used (1). MLP is a synthetic promoter with three complementing sites for the gRNA (2).

Characterization

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 250
1000
COMPATIBLE WITH RFC[1000]

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 ===Usage and Biology===
-gRNA is part of CRISPR/Cas9 complex. guide RNA is a hundred base-long molecule with a unique two dimensional structure which binds Cas9 and guides it to a dsDNA sequence complementary to 21-22 base pairs on the 5' end of the molecule. gRNA scaffold sequence for SaCas9 was used (1).  MLP is a synthetic promoter with three complementing sites for the gRNA.
+gRNA is part of CRISPR/Cas9 complex. guide RNA is a hundred base-long molecule with a unique two dimensional structure which binds Cas9 and guides it to a dsDNA sequence complementary to 21-22 base pairs on the 5' end of the molecule. gRNA scaffold sequence for SaCas9 was used (1).  MLP is a synthetic promoter with three complementing sites for the gRNA (2).
 ===Characterization===