Difference between revisions of "Part:BBa K1699004:Design"
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===Source=== | ===Source=== | ||
| − | + | gRNA for UBB by IDT de-novo syntesis. | |
===References=== | ===References=== | ||
1. In vivo genome editing using Staphylococcus aureus Cas9. | 1. In vivo genome editing using Staphylococcus aureus Cas9. | ||
<br />http://10.1038/nature14299 | <br />http://10.1038/nature14299 | ||
Revision as of 19:57, 5 September 2015
gRNA for SaCas9 targeting human ubiquitin B gene under U6 promoter
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 250
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Cloned into pSBC13 using EcoRI and PstI restriction sites.
F. tagtcatggcggccgcgtcgacG
R. actgacatgcggccgcttaattaaCTGC
Source
gRNA for UBB by IDT de-novo syntesis.
References
1. In vivo genome editing using Staphylococcus aureus Cas9.
http://10.1038/nature14299