Difference between revisions of "Part:BBa K1699005"
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===Usage and Biology=== | ===Usage and Biology=== | ||
| − | gRNA is part of CRISPR/Cas9 complex. MLP is a synthetic promoter with three complementing sites for the gRNA. | + | gRNA is part of CRISPR/Cas9 complex. guide RNA is a hundred base-long molecule with a unique two dimensional structure which binds Cas9 and guides it to a dsDNA sequence complementary to 21-22 base pairs on the 5' end of the molecule. gRNA scaffold sequence for SaCas9 was used (1). MLP is a synthetic promoter with three complementing sites for the gRNA. |
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===Characterization=== | ===Characterization=== | ||
Revision as of 19:59, 5 September 2015
gRNA for dCas9-VP64 targeting synthetic activation promoter pMLPm under U6 promoter
gRNA for dCas9-VP64 targeting synthetic activation promoter pMLPm under U6(RNA pol III) promoter.
Usage and Biology
gRNA is part of CRISPR/Cas9 complex. guide RNA is a hundred base-long molecule with a unique two dimensional structure which binds Cas9 and guides it to a dsDNA sequence complementary to 21-22 base pairs on the 5' end of the molecule. gRNA scaffold sequence for SaCas9 was used (1). MLP is a synthetic promoter with three complementing sites for the gRNA.
Characterization
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 250
- 1000COMPATIBLE WITH RFC[1000]