Difference between revisions of "Part:BBa K1699002:Design"

(→‎Design Notes)
(→‎Design Notes)
Line 8: Line 8:
  
 
Cloned into pSB1C3 using EcoRI and PstI restriction sites.
 
Cloned into pSB1C3 using EcoRI and PstI restriction sites.
 +
F. tagtcatgGAATTCGCGGCCGCTTCTAG
 +
R. tgtatactCTGCAGCGGCCGCTACTAG
  
 
===Source===
 
===Source===

Revision as of 19:07, 5 September 2015

gRNA for SaCas9 targeting human ubiquitin B gene


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 146
    Illegal NgoMIV site found at 175
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Cloned into pSB1C3 using EcoRI and PstI restriction sites. F. tagtcatgGAATTCGCGGCCGCTTCTAG R. tgtatactCTGCAGCGGCCGCTACTAG

Source

IDT de-novo synthesis.

References

1. Self-processing of ribozyme-flanked RNAs into guide RNAs in vitro and in vivo for CRISPR-mediated genome editing http://onlinelibrary.wiley.com/doi/10.1111/jipb.12152/full