Difference between revisions of "Part:BBa K1824555"

Revision as of 20:39, 4 September 2015

Site-directed mutated pLux that permits strong validity

This is a mutated pLux that permits ideal ON/OFF ratio based on the original promoter Lux pR BBa_R0062. BBa_R0062 was believed to have high constitutative expression in the absent of 3-oxo-hexanoyl-HSL and this problem went serious when regulatory promoter was coupled with toxins or other repression/activation parts. By using this mutated pLux, genetic circuits would be more easily to control and more sensitive to external signal change.

The credit for designing this part should be assigned to Haoqian Zhang, Peking University.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K1824555 short</partinfo>
-This is a mutated pLux that permits ideal ON/OFF ratio based on the original promoter Lux pR (BBa_R0062). BBa_R0062 was believed to have high constitutative expression in the absent of 3-oxo-hexanoyl-HSL and this problem went serious when regulatory promoter was coupled with toxins or other repression/activation parts. By using this mutated pLux, genetic circuits would be more easily to control and more sensitive to external signal change.
+This is a mutated pLux that permits ideal ON/OFF ratio based on the original promoter Lux pR <partinfo>BBa_R0062</partinfo>. BBa_R0062 was believed to have high constitutative expression in the absent of 3-oxo-hexanoyl-HSL and this problem went serious when regulatory promoter was coupled with toxins or other repression/activation parts. By using this mutated pLux, genetic circuits would be more easily to control and more sensitive to external signal change.
 The credit for designing this part should be assigned to Haoqian Zhang, Peking University.