Difference between revisions of "Part:BBa K1620007"
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<partinfo>BBa_K1620007 short</partinfo> | <partinfo>BBa_K1620007 short</partinfo> | ||
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
<partinfo>BBa_K1620007 SequenceAndFeatures</partinfo> | <partinfo>BBa_K1620007 SequenceAndFeatures</partinfo> | ||
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<!-- Uncomment this to enable Functional Parameter display | <!-- Uncomment this to enable Functional Parameter display | ||
===Functional Parameters=== | ===Functional Parameters=== | ||
<partinfo>BBa_K1620007 parameters</partinfo> | <partinfo>BBa_K1620007 parameters</partinfo> | ||
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+ | ==Usage and Biology== | ||
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+ | <p align="justify"> | ||
+ | SmtA is a metallothionein which bind to divalent metallic cations such as Zn(II). This SmtA was an improvement of the biobrick <partinfo>BBa_K519010</partinfo> originally cloned from Synechococcus sp. PCC7942, a cyanobacterial strain. SmtA is a low cys content type II metallothionein. Other team (iGEM11_Tokyo-NoKoGen, 2011-09-30) have previously find SmtA constitutively expressed in E. coli improving its growth at a high Cd(II) concentrationwhen compared to wild type E. coli. This part was designed to eliminate an internal restriction site for PstI, the 98th base was changed G -> A. No residues were changed. | ||
+ | </p> | ||
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+ | [[File: Alignment between improved SmtA and BBa_K519010.jpg|450px|thumb|left|'''Figure 1:''' Alignment between improved SmtA and Bba_K519010.]] | ||
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Revision as of 13:20, 5 September 2015
Synechococcus metallothionenin A (SmtA)
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 121
- 1000COMPATIBLE WITH RFC[1000]
Usage and Biology
SmtA is a metallothionein which bind to divalent metallic cations such as Zn(II). This SmtA was an improvement of the biobrick BBa_K519010 originally cloned from Synechococcus sp. PCC7942, a cyanobacterial strain. SmtA is a low cys content type II metallothionein. Other team (iGEM11_Tokyo-NoKoGen, 2011-09-30) have previously find SmtA constitutively expressed in E. coli improving its growth at a high Cd(II) concentrationwhen compared to wild type E. coli. This part was designed to eliminate an internal restriction site for PstI, the 98th base was changed G -> A. No residues were changed.