Difference between revisions of "Part:BBa K1720002:Design"

(Design Notes:)
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===Design Notes:===
 
We cloned this basic part into a psb1c3 vector, this part contain a hypoxia-responsive element and CMV promoter.The hypoxia-responsive element is a minimalcis-regulatory element mediating transactivation by the hypoxia-inducible factor (HIF) in mammalian.So that this part only applies to mammalian organism.
 
  
  
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===Design Notes:===
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We cloned this basic part into a psb1c3 vector, this part contain a hypoxia-responsive element and CMV promoter.The hypoxia-responsive element is a minimalcis-regulatory element mediating transactivation by the hypoxia-inducible factor (HIF) in mammalian.So that this part only applies to mammalian organism.
  
 
===References===
 
===References===

Revision as of 13:21, 4 September 2015


Design Notes:

We cloned this basic part into a psb1c3 vector, this part contain a hypoxia-responsive element and CMV promoter.The hypoxia-responsive element is a minimalcis-regulatory element mediating transactivation by the hypoxia-inducible factor (HIF) in mammalian.So that this part only applies to mammalian organism.

References

1.Rational design of minimal hypoxia-inducible enhancers Stefan Kaluz*, Milota Kaluzová, Eric J. Stanbridge Department of Microbiology and Molecular Genetics, Medical Science I, B210, University of California at Irvine, College of Medicine, CA 92697-4025, USA