Difference between revisions of "Part:BBa K1742001:Design"

 
(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
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The protein consists of the E. coli enterotoxin beta subunit. It is codon optimized for plants and contains a C-terminus 6X Hys tag in order to detected by anti-Hys antibodies.
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The coding sequence of the E.coli heat labile enterotoxin beta subunit (LTB) was obtained by gene synthesis (Integrated DNA technology, IDT) and adapted to the coding sequence of Nicotiana tabacum
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===Source===
 
===Source===

Revision as of 23:31, 16 September 2015


Entertoxin LTB


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The coding sequence of the E.coli heat labile enterotoxin beta subunit (LTB) was obtained by gene synthesis (Integrated DNA technology, IDT) and adapted to the coding sequence of Nicotiana tabacum

Source

The LTB protein was designed based on a publication that showed that it can be expressed in Nicotiana Benthamiana plants.

References