Difference between revisions of "Part:BBa K1620001"
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− | + | ===Usage and Biology=== | |
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− | < | + | It is a zinc activity sensing promoter element. The regulation of this promoter is Zur dependent and positive in absent of zinc or other divalent metallic cations in medium. The Zur protein binds those cations repressing the expression of the gene downstream Zasp element. In our project, it was used to trigger the expression of killer genes downstream this element. Since ZnuABC activity is extremely sensitive to low zinc concentrations, we believe that this component will be useful for other teams in order to its inducible feature and precise functioning. |
+ | </p> |
Latest revision as of 21:24, 13 September 2015
Promoter element Zasp
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Usage and Biology
It is a zinc activity sensing promoter element. The regulation of this promoter is Zur dependent and positive in absent of zinc or other divalent metallic cations in medium. The Zur protein binds those cations repressing the expression of the gene downstream Zasp element. In our project, it was used to trigger the expression of killer genes downstream this element. Since ZnuABC activity is extremely sensitive to low zinc concentrations, we believe that this component will be useful for other teams in order to its inducible feature and precise functioning.