Difference between revisions of "Part:BBa K1699003"
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<partinfo>BBa_K1699003 short</partinfo>
 
<partinfo>BBa_K1699003 short</partinfo>
  
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Ribozyme flanked gRNA compatible for dCas9-VP64. gRNA sequence complements 3 different loci in the synthetic promoter pMLPm, and gRNA dCas9 complex can promote transcription downstream of syntetic promoter (9). It has a hammerhead ribozyme on its 5' and an HDV ribozyme on its 3' end. Upon transcription the ribozymes should cleave the mRNA at specific locations to release the mature gRNA (6).
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Ribozyme flanked gRNA compatible for dCas9-VP64. gRNA sequence complements 3 different loci in the synthetic promoter pMLPm, and gRNA dCas9 complex can promote transcription downstream of syntetic promoter. It has a hammerhead ribozyme on its 5' and an HDV ribozyme on its 3' end. Upon transcription the ribozymes should cleave the mRNA at specific locations to release the mature gRNA.
  
  
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<partinfo>BBa_K1699003 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1699003 SequenceAndFeatures</partinfo>
  
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===References===
 
  
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6. Self-processing of ribozyme-flanked RNAs into guide RNAs in vitro and in vivo for CRISPR-mediated genome editing
 
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http://onlinelibrary.wiley.com/doi/10.1111/jipb.12152/full
 
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9. Tunable and Multifunctional Eukaryotic Transcription Factors Based on CRISPR/Cas
 
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http://pubs.acs.org/doi/full/10.1021/sb400081r
 
  
 
<!-- Uncomment this to enable Functional Parameter display  
 
<!-- Uncomment this to enable Functional Parameter display  

Revision as of 08:27, 3 September 2015

gRNA for dCas9-VP64 targeting synthetic activation promoter pMLPm

Ribozyme flanked gRNA compatible for dCas9-VP64. gRNA sequence complements 3 different loci in the synthetic promoter pMLPm, and gRNA dCas9 complex can promote transcription downstream of syntetic promoter. It has a hammerhead ribozyme on its 5' and an HDV ribozyme on its 3' end. Upon transcription the ribozymes should cleave the mRNA at specific locations to release the mature gRNA.


Usage and Biology

usage and biology


Characterization

characterization

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 144
    Illegal NgoMIV site found at 173
  • 1000
    COMPATIBLE WITH RFC[1000]