Difference between revisions of "Part:BBa K1613018:Design"

 
(→‎Source)
Line 13: Line 13:
 
===Source===
 
===Source===
  
−
using part: K1555000(promoter+rbs)
+
using part: CopA  (promoter)
 +
         
 +
          B0030  (RBS)
  
−
           E1010  (Red Flourescent Protein)
+
           E1010  (RFP)
  
−
           B0015  (Terminator)
+
           B0015  (Terminator)
  
 
===References===
 
===References===

Revision as of 06:14, 6 September 2015


Device with CopA promoter to detect copper


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 882
    Illegal AgeI site found at 994
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

When connection k1555000 and RFP, there was no capable restriction enzyme to use. So we need to add capable restriction enzyme on primer to solve it.


Source

using part: CopA (promoter) 

          B0030   (RBS)

          E1010   (RFP)

          B0015   (Terminator)

References