Difference between revisions of "Part:BBa K1618022"

 
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<partinfo>BBa_K1618022 short</partinfo>
 
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GlgB is a genetic sequence which codes for a glycogen branching enzyme which adds branches to glycogen strands to make the entire molecule more branched. The coding sequence is preceded by an IPTG-inducible promoter and both sequences are encoded within the pSB1C3 vector.
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GlgB is a genetic sequence which codes for a glycogen branching enzyme which adds branches onto glycogen strands. The enzyme cleabes alpha-1,4 linkages in glycogen strands and re-anneals the cleaved strand back onto the main glycogen strand through an alpha-1,6 linkage. This therefore creates the branch. The coding sequence is preceded by an IPTG-inducible promoter and both sequences are encoded within the pSB1C3 vector. The bacteria therefore shows chloramphenicol resistance.
  
 
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Revision as of 14:31, 9 September 2015

GlgB with IPTG-inducible promoter

GlgB is a genetic sequence which codes for a glycogen branching enzyme which adds branches onto glycogen strands. The enzyme cleabes alpha-1,4 linkages in glycogen strands and re-anneals the cleaved strand back onto the main glycogen strand through an alpha-1,6 linkage. This therefore creates the branch. The coding sequence is preceded by an IPTG-inducible promoter and both sequences are encoded within the pSB1C3 vector. The bacteria therefore shows chloramphenicol resistance.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 283
    Illegal BamHI site found at 1491
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 917