Difference between revisions of "Part:BBa K1758351:Design"
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− | |||
__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K1758351 short</partinfo> | <partinfo>BBa_K1758351 short</partinfo> | ||
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===Design Notes=== | ===Design Notes=== | ||
− | ... | + | <html> |
+ | We used the codon optimized, synthesized Sequence of RFP under the control of rcnA from iGEM Team BIT 2013(<a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K549001" target="_blank"> BBa_K549001 </a>). We designed the used primes, in specially split primers, for Gibson Assembly to ligate the RFP under control of rcnA with pSB1C3. For this aim we designed four primers for the generation of homologous overlaps between the synthesized RFP under control of rcnA and the pSB1C3: | ||
+ | <p><a href="http://2015.igem.org/Team:Bielefeld-CeBiTec/Primers#cm_fwd" target="_blank"> <a href="http://2015.igem.org/Team:Bielefeld-CeBiTec/Primers#cm_fwd" target="_blank">cm_fwd</a></p> | ||
+ | <p> CGGCATCAGCACCTTGTC </p> | ||
+ | |||
+ | <p><a href="http://2015.igem.org/Team:Bielefeld-CeBiTec/Primers# pSB1C3_RFP_rev" target="_blank"> <a href="http://2015.igem.org/Team:Bielefeld-CeBiTec/Primers# pSB1C3_RFP_rev" target="_blank"> pSB1C3_RFP_rev</a></p> | ||
+ | <p>CCGCCACTCAACGGGTGCCTGATAATACTAGTAGCGGCCGCTGCA</p> | ||
+ | |||
+ | <p><a href="http://2015.igem.org/Team:Bielefeld-CeBiTec/Primers#cm_rev" target="_blank"> <a href="http://2015.igem.org/Team:Bielefeld-CeBiTec/Primers#cm_rev" target="_blank">cm_rev</a></p> | ||
+ | <p> TATACGCAAGGCGACAAG </p> | ||
+ | <p><a href="http://2015.igem.org/Team:Bielefeld-CeBiTec/Primers#pSB1C3_rcnA_fwd" target="_blank"> <a href="http://2015.igem.org/Team:Bielefeld-CeBiTec/Primers# pSB1C3_rcnA_fwd" target="_blank"> pSB1C3_rcnA_fwd</a> | ||
+ | </p> <p>TAGATTAATAGTGCTATGATTTTTTATTGTTAACCAGGTGTTGCCATGTCTCATCTCTAGAAGCGGCCGCGAAT</p> | ||
+ | </html> | ||
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===Source=== | ===Source=== | ||
− | design based on | + | design based on BBa_K549001(<a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K549001" target="_blank"> BBa_K59001</a>) |
===References=== | ===References=== |
Latest revision as of 22:59, 18 September 2015
Nickel responsive promoter with RFP
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We used the codon optimized, synthesized Sequence of RFP under the control of rcnA from iGEM Team BIT 2013( BBa_K549001 ). We designed the used primes, in specially split primers, for Gibson Assembly to ligate the RFP under control of rcnA with pSB1C3. For this aim we designed four primers for the generation of homologous overlaps between the synthesized RFP under control of rcnA and the pSB1C3:
CGGCATCAGCACCTTGTC
CCGCCACTCAACGGGTGCCTGATAATACTAGTAGCGGCCGCTGCA
TATACGCAAGGCGACAAG
TAGATTAATAGTGCTATGATTTTTTATTGTTAACCAGGTGTTGCCATGTCTCATCTCTAGAAGCGGCCGCGAAT
Source
design based on BBa_K549001(<a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K549001" target="_blank"> BBa_K59001</a>)