Difference between revisions of "Part:BBa K1758321:Design"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K1758321 short</partinfo> | <partinfo>BBa_K1758321 short</partinfo> | ||
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===Design Notes=== | ===Design Notes=== | ||
− | --- | + | <html> |
+ | We used the codon optimized, synthesized Sequence of RFP under the control of <i>copAP </i> from <i> E.coli </i> K12. We designed the used primes, in specially split primers, for Gibson Assembly to ligate the RFP under control of <i>copAP</i> with pSB1C3. For this aim we designed four primers for the generation of homologous overlaps between the synthesized RFP under control of <i> copAP</i> and the pSB1C3: | ||
+ | <p><a href="http://2015.igem.org/Team:Bielefeld-CeBiTec/Primers#cm_fwd" target="_blank"> <a href="http://2015.igem.org/Team:Bielefeld-CeBiTec/Primers#cm_fwd" target="_blank">cm_fwd</a></p> | ||
+ | <p>CGGCATCAGCACCTTGTC </p> | ||
+ | <p><a href="http://2015.igem.org/Team:Bielefeld-CeBiTec/Primers# pSB1C3_RFP_rev" target="_blank"> <a href="http://2015.igem.org/Team:Bielefeld-CeBiTec/Primers# pSB1C3_RFP_rev" target="_blank"> pSB1C3_RFP_rev</a> </p> | ||
+ | <p> CCGCCACTCAACGGGTGCCTGATAATACTAGTAGCGGCCGCTGCA </p> | ||
+ | <p><a href="http://2015.igem.org/Team:Bielefeld-CeBiTec/Primers#cm_rev" target="_blank"> <a href="http://2015.igem.org/Team:Bielefeld-CeBiTec/Primers#cm_rev" target="_blank">cm_rev</a> </p> | ||
+ | <p> TATACGCAAGGCGACAAG </p> | ||
+ | <p><a href="http://2015.igem.org/Team:Bielefeld-CeBiTec/Primers#pSB1C3_copAP_fwd" target="_blank"> <a href="http://2015.igem.org/Team:Bielefeld-CeBiTec/Primers# pSB1C3_copAP_fwd" target="_blank"> pSB1C3_copAP_fwd</a></p> | ||
+ | <p>TTGACCTTCCCCTTGCTGGAAGGTTTAACCTTTATCACAATTAAAGAGGAGAAATACTAG</p> | ||
+ | </html> | ||
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+ | ===Source=== | ||
+ | <html> | ||
+ | <i>CopAp</i> is a copper induceble promoter out of <i>E.coli</i> K12 | ||
+ | </html> | ||
===References=== | ===References=== |
Revision as of 03:09, 19 September 2015
mRFP under control of copper responsive promoter
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We used the codon optimized, synthesized Sequence of RFP under the control of copAP from E.coli K12. We designed the used primes, in specially split primers, for Gibson Assembly to ligate the RFP under control of copAP with pSB1C3. For this aim we designed four primers for the generation of homologous overlaps between the synthesized RFP under control of copAP and the pSB1C3:
CGGCATCAGCACCTTGTC
CCGCCACTCAACGGGTGCCTGATAATACTAGTAGCGGCCGCTGCA
TATACGCAAGGCGACAAG
TTGACCTTCCCCTTGCTGGAAGGTTTAACCTTTATCACAATTAAAGAGGAGAAATACTAG
Source
CopAp is a copper induceble promoter out of E.coli K12