Difference between revisions of "Part:BBa K1621007:Design"

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<partinfo>BBa_K1621007 short</partinfo>
 
 
<partinfo>BBa_K1621007 SequenceAndFeatures</partinfo>
 
 
 
===Design Notes===
 
At position 15 a base change from guanine to thymine was inserted by site-directed mutagenesis to remove the recognition site for PstI without causing a change in the amino acid sequence.
 
 
 
 
===Source===
 
 
The part's sequence as well as an expression plasmid carrying a His-tagged variant was obtained from the group of Prof. Dr. Hust (TU Braunschweig).
 
 
===References===
 
 
Meyer T, Schirrmann T, Frenzel A, Miethe S, Stratmann-Selke J, Gerlach GF, Strutzberg-Minder K, Dübel S, Hust M: Identification of immunogenic proteins and generation of antibodies against ''Salmonella'' Typhimurium using phage display (2012). ''BMC Biotechnology'' 12:29.
 

Latest revision as of 15:23, 26 October 2015