Difference between revisions of "Part:BBa K1602000"
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         <h1>Inducible itaconic acid producing operon</h1>
 
         <h1>Inducible itaconic acid producing operon</h1>
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         <b>Itaconic acid</b> is an organic, dicarboxylic acid that is biotechnologically synthesized in Aspergillus terreus. It is derived from citric acid             via 2 intermediates and a final decarboxylation.
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         <b>Itaconic acid</b> is an organic, dicarboxylic acid that is biotechnologically synthesized most commonly in Aspergillus terreus. It is derived           from citric acid via 2 intermediates and a final decarboxylation.
 
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         To enable this pathway in <i>Escherichia coli</i> it is necessary to introduce 3 genes. 2 of which are already established in the citric acid cycle            and one that is from <i>Apergillus terreus</i>, a cis-aconitate decarboxylase (cadA).
 
         To enable this pathway in <i>Escherichia coli</i> it is necessary to introduce 3 genes. 2 of which are already established in the citric acid cycle            and one that is from <i>Apergillus terreus</i>, a cis-aconitate decarboxylase (cadA).

Revision as of 16:55, 28 August 2015

Inducible itaconic acid producing operon

Itaconic acid is an organic, dicarboxylic acid that is biotechnologically synthesized most commonly in Aspergillus terreus. It is derived from citric acid via 2 intermediates and a final decarboxylation.
To enable this pathway in Escherichia coli it is necessary to introduce 3 genes. 2 of which are already established in the citric acid cycle and one that is from Apergillus terreus, a cis-aconitate decarboxylase (cadA).

Figure 1 Reaction scheme of the itaconic acid producing operon. The substrates for the reaction are acetyl-coa and oxaloacetate. To simplify the process we are only looking at oxaloacetate right now. Oxaloacetate is metabolized to itaconic acid in 3 steps.



Usage

This part is a composite of three genes, each with a strong RBS (BBa_B0034) and all of them under control of one T7 Promoter (BBa_K1497017).


Figure 2 Genetic map of the itaconic acid producing operon with T7 promoter. This brick directs the flux towards and finally enables E.Coli BL21 cells to synthesize itaconic acid in presence of the inductor IPTG.