Difference between revisions of "Part:BBa K1602000"
Line 1: Line 1:
−
__NOTOC__
+
<!DOCTYPE html>
−
<partinfo>BBa_K1602000 short</partinfo>
+
<html>
−
 
+
    <head>
−
<style type="text/css">
+
        <style>
−
<!--
+
            table, td, th { border: 0px solid black; }
−
 
+
        </style>
−
  ul.aufz10 {
+
    </head>
−
 
+
    <body>
−
  type="circle";
+
        <h1>Inducible itaconic acid producing operon</h1>
−
 
+
        <b>Itaconic acid</b> is an organic, dicarboxylic acid that is biotechnologically synthesized in Aspergillus terreus. It is derived from citric acid             via 2 intermediates and a final decarboxylation.
−
  padding: 10px;
+
        <br>
−
 
+
        To enable this pathway in <i>Escherichia coli</i> it is necessary to introduce 3 genes. 2 of which are already established in the citric acid cycle           and one that is from <i>Apergillus terreus</i>, a cis-aconitate decarboxylase (cadA).
−
  margin: 15px 0px 0px 25px;
+
        <br>
−
 
+
        <br>
−
  }
+
        <img style="width: 900px; height: 110px; margin-left: 15px; margin-right: 15px;" alt="" src="pictures/chem_way.jpg">
−
 
+
        <p style="width: 900px; margin-left: 15px; margin-right: 15px;" alt="">
−
  .block-10vi {
+
            <b>Figure 1</b> Reaction scheme of the itaconic acid producing operon. The substrates for the reaction are acetyl-coa and oxaloacetate. To                     simplify the process we are only looking at oxaloacetate right now. Oxaloacetate is metabolized to itaconic acid in 3 steps.
−
 
+
        </p>
−
  list-style-image: url(https://static.igem.org/mediawiki/parts/5/57/Bild5.png);
+
        <br>
−
 
+
        <br>
−
  font-family: Verdana,sans-serif;
+
        <h2>Usage</h2>
−
 
+
            This part is a composite of three genes, each with a strong RBS (<a href="/Part:BBa_B0034">BBa_B0034</a>) and all of them under control of one             T7 Promoter (<a href="/Part:BBa_K1497017">BBa_K1497017</a>).
−
  font-size: 12px;
+
        <br>
−
 
+
        <br>
−
  font-weight: normal;
+
        <br>
−
 
+
        <table>
−
  color: #000000;
+
            <colgroup width=50% span="2">
−
 
+
            <tr>
−
  }
+
                <td>
−
 
+
                    <ul>
−
-->
+
                        <li class="block-10vi">citrate synthase - gltA                     <a href="/Part:BBa_K1033001">(BBa_K1033001)</a></li>
−
</style>
+
                        <li class="block-10vi">aconitate hydratase 1 - acnA             <a href="/Part:BBa_K1033000">(BBa_K1033000)</a></li>
−
 
+
                        <li class="block-10vi">cis-aconitate decarboxylase - cadA            <a href="/Part:BBa_K1497000">(BBa_K1497100)</a></li>  
−
<partinfo>BBa_IA</partinfo>
+
                    </ul>
−
 
+
                </td>
−
  <html>
+
                <td>
−
 
+
                    <div style="float: right;"><img style="width: 400px; height: 120px;" alt="" src="pictures/gene_operon.png"> </div>     
−
     
+
                </td>
−
 
+
            </tr>
−
<b>Itaconic acid</b> is an organic, dicarboxylic acid that is biotechnologically synthesized in Aspergillus terreus. It is derived from citric acid         via 2 intermediates and a final decarboxylation.
+
            <tr>
−
 
+
                <td>
−
     
+
                    <!-- BLANK -->
−
 
+
                </td>
−
To enable this pathway in Escherichia coli it is necessary to introduce 3 genes. 2 of which are already established in the citric acid cycle and           one that is from Apergillus terreus, a cis-aconitate decarboxylase (cadA).
+
                <td>
−
 
+
                    <b>Figure 2</b> Genetic map of the itaconic acid producing operon with T7 promoter. This brick directs the flux towards and finally                           enables <i>E.Coli</i> BL21 cells to synthesize itaconic acid in presence of the inductor IPTG.
−
          <img style="width: 900px; height: 99,4px; margin-left: 15px; margin-right: 15px;" alt=""  
+
                </td>
−
 
+
            </tr>
−
          src="pictures/operon_ia.001.jpg">
+
            </colgroup>
−
 
+
        </table>
−
          <p style="width: 900px; height: 99,4px; margin-left: 15px; margin-right: 15px;" alt="" >
+
       
−
 
+
    </body>
−
     
+
</html>
−
 
+
−
<b>Figure 1</b> Reaction scheme of the itaconic acid producing operon. The substrates for the reaction are acetyl-coa and oxaloacetate. To                 simplify the process we are only looking at oxaloacetate right now. Oxaloacetate is metabolized to itaconic acid in 3 steps.
+
−
 
+
−
         
+
−
 
+
−
     
+
−
 
+
−
===Usage===
+
−
 
+
−
<div align="right">
+
−
<table class="MsoTableGrid"
+
−
 
+
−
style="border: medium none ; border-collapse: collapse; text-align: left; margin-left: auto; margin-right: auto;"
+
−
 
+
−
border="0" cellpadding="0" cellspacing="0">
+
−
 
+
−
<tbody>
+
−
 
+
−
  <tr style="height: 214.9pt;">
+
−
 
+
−
   
+
−
 
+
−
      <td style="padding: 0cm 5.4pt; width: 336.7pt; height: 214.9pt;"> (Herr Pilger ist doof!) This part is a composite of three genes, each with a strong RBS (<a                       href="/Part:BBa_B0034">BBa_B0034</a>) and all of them under control of one T7 Promoter (<a href="/Part:BBa_K1497017">BBa_K1497017</a>).
+
−
 
+
−
     
+
−
 
+
−
<ul class="aufz10">
+
−
 
+
−
              <li class="block-10vi">citrate synthase - gltA                     <a href="/Part:BBa_K1033001">(BBa_K1033001)</a></li>
+
−
 
+
−
              <li class="block-10vi">aconitate hydratase 1 - acnA             <a href="/Part:BBa_K1033000">(BBa_K1033000)</a></li>
+
−
 
+
−
              <li class="block-10vi">cis-aconitate decarboxylase - cadA            <a href="/Part:BBa_K1497000">(BBa_K1497100)</a></li>  
+
−
 
+
−
          </ul>
+
−
 
+
−
      </td>
+
−
 
+
−
      <td>  
+
−
 
+
−
          <font color="#FFFFFF">iGEM TU Darmstadt 2014 :)</font>
+
−
 
+
−
</td>
+
−
 
+
−
      <td style="padding: 0cm 5.4pt; vertical-align: top; width: 236.7pt; height: 214.9pt;">
+
−
 
+
−
          <img style="width: 400px; height: 120px;" alt="" src="pictures/all.png">
+
−
 
+
−
         
+
−
 
+
−
   
+
−
 
+
−
          <p class="MsoCaption" align="text-align:justify">
+
−
 
+
−
              <b>Figure 2</b>
+
−
 
+
−
              Genetic map of the itaconic acid producing operon with T7 promoter. This brick directs the flux towards and finally enables <i>E.Coli</i>                   BL21 cells to synthesize itaconic acid in presence of the inductor IPTG.
+
−
 
+
−
         
+
−
 
+
−
</td>
+
−
 
+
−
  </tr>
+
−
</tbody>
+
−
</table>
+
−
</div>
+
−
 
+
−
 
+
−
 
+
−
<!-- Add more about the biology of this part here
+
−
===Usage and Biology===
+
−
 
+
−
<!-- -->
+
−
<span class='h3bb'>Sequence and Features</span>
+
−
<partinfo>BBa_K1602000 SequenceAndFeatures</partinfo>
+
−
 
+
−
 
+
−
<!-- Uncomment this to enable Functional Parameter display
+
−
===Functional Parameters===
+
−
<partinfo>BBa_K1602000 parameters</partinfo>
+
−
<!-- -->
+

Revision as of 16:44, 28 August 2015

<!DOCTYPE html>

Inducible itaconic acid producing operon

Itaconic acid is an organic, dicarboxylic acid that is biotechnologically synthesized in Aspergillus terreus. It is derived from citric acid via 2 intermediates and a final decarboxylation.
To enable this pathway in Escherichia coli it is necessary to introduce 3 genes. 2 of which are already established in the citric acid cycle and one that is from Apergillus terreus, a cis-aconitate decarboxylase (cadA).

Figure 1 Reaction scheme of the itaconic acid producing operon. The substrates for the reaction are acetyl-coa and oxaloacetate. To simplify the process we are only looking at oxaloacetate right now. Oxaloacetate is metabolized to itaconic acid in 3 steps.



Usage

This part is a composite of three genes, each with a strong RBS (BBa_B0034) and all of them under control of one T7 Promoter (BBa_K1497017).


Figure 2 Genetic map of the itaconic acid producing operon with T7 promoter. This brick directs the flux towards and finally enables E.Coli BL21 cells to synthesize itaconic acid in presence of the inductor IPTG.