Difference between revisions of "Part:BBa K1758106"
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<partinfo>BBa_K1758106 short</partinfo> | <partinfo>BBa_K1758106 short</partinfo> | ||
| − | mRFP | + | mRFP ([https://parts.igem.org/Part:BBa_K283035 BBa_K283035]) under control of the T7 promoter ([https://parts.igem.org/Part:BBa_I719005 BBa_I719005]). The part includes a translation enhancing sequence, 5'-UTR, which improves the binding of the mRNA to the ribosome ([http://www.ncbi.nlm.nih.gov/pubmed/2676996 Olins et al. 1989]). The 5'-UTR sequence contains a spacer of 10 adenine bases which has been shown to further enhance translation efficiency ([http://www.ncbi.nlm.nih.gov/pubmed/23927491 Takahashi et al. 2013]). |
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Revision as of 23:47, 27 August 2015
Translation enhancing 5-UTR + mRFP under control of T7 promoter
mRFP (BBa_K283035) under control of the T7 promoter (BBa_I719005). The part includes a translation enhancing sequence, 5'-UTR, which improves the binding of the mRNA to the ribosome ([http://www.ncbi.nlm.nih.gov/pubmed/2676996 Olins et al. 1989]). The 5'-UTR sequence contains a spacer of 10 adenine bases which has been shown to further enhance translation efficiency ([http://www.ncbi.nlm.nih.gov/pubmed/23927491 Takahashi et al. 2013]).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 624
Illegal AgeI site found at 736 - 1000COMPATIBLE WITH RFC[1000]