Difference between revisions of "Part:BBa K1758105"

 
 
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<partinfo>BBa_K1758105 short</partinfo>
 
<partinfo>BBa_K1758105 short</partinfo>
  
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mRFP is synthesized when T7-polymerase is induced.
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mRFP ([https://parts.igem.org/Part:BBa_K283035 BBa_K283035]) under control of the T7 promoter ([https://parts.igem.org/Part:BBa_I719005 BBa_I719005]). This part was used to create ([https://parts.igem.org/Part:BBa_K1758106 BBa_K1758106].
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<!-- Add more about the biology of this part here
 
 
===Usage and Biology===
 
===Usage and Biology===
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<html>
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<p>mRFP is synthesized when T7-polymerase is present. This part is a mRFP generator.</p>
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<h2> Results </h2>
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<p> This part was used to charactize the effect of our designed, translation enhancing 5'-untranslated region (5'-UTR; <a href="https://parts.igem.org/Part:BBa_K1758100">BBa_K1758100</a>), on protein production. </p>
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<figure>
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<a href="https://static.igem.org/mediawiki/parts/7/7e/Bielefeld-CeBiTec_UTR_characterization_in_vivo_mrfp.jpg"><img src="https://static.igem.org/mediawiki/parts/7/7e/Bielefeld-CeBiTec_UTR_characterization_in_vivo_mrfp.jpg" alt="cultures of mRFP producting cells" style="width:300px"></a>
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<figcaption> Image of mRFP producing <i>E. coli</i> cultures. Left: <a href="https://parts.igem.org/Part:BBa_K1758106">BBa_K1758106</a>, right: This part. Both cultures were cultivated for the same time span. You can see that our designed, translation enhancing 5'-untranslated region (5'-UTR; <a href="https://parts.igem.org/Part:BBa_K1758100">BBa_K1758100</a>), improves mRFP production <i>in vivo</i>. See <a href="https://parts.igem.org/Part:BBa_K1758106">BBa_K1758106</a> for further details.</figcaption>
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</figure>
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</html>
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Latest revision as of 18:53, 19 September 2015

mRFP generator under control of T7 promoter

mRFP (BBa_K283035) under control of the T7 promoter (BBa_I719005). This part was used to create (BBa_K1758106.


Usage and Biology

mRFP is synthesized when T7-polymerase is present. This part is a mRFP generator.

Results

This part was used to charactize the effect of our designed, translation enhancing 5'-untranslated region (5'-UTR; BBa_K1758100), on protein production.

cultures of mRFP producting cells
Image of mRFP producing E. coli cultures. Left: BBa_K1758106, right: This part. Both cultures were cultivated for the same time span. You can see that our designed, translation enhancing 5'-untranslated region (5'-UTR; BBa_K1758100), improves mRFP production in vivo. See BBa_K1758106 for further details.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 607
    Illegal AgeI site found at 719
  • 1000
    COMPATIBLE WITH RFC[1000]