Difference between revisions of "Part:BBa M13008"
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This gene is part of the bacteriophage M13 genome. It encodes the major coat protein, with approximately 2700 copies of the protein on each phage particle. The protein is first synthesized at a longer precursor protein that is clipped between the Alanines at positions 23 and 24. It is possible to silently add a Pst site to clone fusions upstream and in frame with Ala that begins mature protein. Small peptide-fusions are possible to the N-terminal portion of the mature p8 (phage display systems are built around this finding) but addition of more than 6 or 8 residues makes the phage pack poorly, leading to unproductive infections. | This gene is part of the bacteriophage M13 genome. It encodes the major coat protein, with approximately 2700 copies of the protein on each phage particle. The protein is first synthesized at a longer precursor protein that is clipped between the Alanines at positions 23 and 24. It is possible to silently add a Pst site to clone fusions upstream and in frame with Ala that begins mature protein. Small peptide-fusions are possible to the N-terminal portion of the mature p8 (phage display systems are built around this finding) but addition of more than 6 or 8 residues makes the phage pack poorly, leading to unproductive infections. | ||
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| + | <strong> Supplementary information by 2020 iGEM Montpellier team</strong> | ||
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| + | https://static.igem.org/mediawiki/parts/b/ba/T--Montpellier--M13schema.png | ||
| + | Figure 1: M13 coat proteins schema. | ||
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| + | The p8 is the major coat protein, wrapping all the filamentous phage. This proteins is very useful and used in phage display when experimenting on phage M13. | ||
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| + | <i>Source:</i> | ||
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| + | W. Markland, B. L. Roberts, M. J. Saxena, S. K. Guterman and R. C. Ladner, Design, construction and function of a multicopy display vector using fusions to the major coat protein of bacteriophage M13, Gene, December 1991. | ||
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Latest revision as of 08:17, 21 October 2020
M13KO7 gene VIII
This gene is part of the bacteriophage M13 genome. It encodes the major coat protein, with approximately 2700 copies of the protein on each phage particle. The protein is first synthesized at a longer precursor protein that is clipped between the Alanines at positions 23 and 24. It is possible to silently add a Pst site to clone fusions upstream and in frame with Ala that begins mature protein. Small peptide-fusions are possible to the N-terminal portion of the mature p8 (phage display systems are built around this finding) but addition of more than 6 or 8 residues makes the phage pack poorly, leading to unproductive infections.
Supplementary information by 2020 iGEM Montpellier team
Figure 1: M13 coat proteins schema.
The p8 is the major coat protein, wrapping all the filamentous phage. This proteins is very useful and used in phage display when experimenting on phage M13.
Source:
W. Markland, B. L. Roberts, M. J. Saxena, S. K. Guterman and R. C. Ladner, Design, construction and function of a multicopy display vector using fusions to the major coat protein of bacteriophage M13, Gene, December 1991.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]