Difference between revisions of "Part:BBa K1606010"
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we modernized m-cherry (BBa_J06504) part to report the absence of the gluten protein in a solution or in a dough that our updated Kumamax enzyme is inactive in the environment. When the absence of the protein of the gluten our repoter gene is expressed and give red light to environment. Besides LacI promoter works under acidic environment in the dough formation. | we modernized m-cherry (BBa_J06504) part to report the absence of the gluten protein in a solution or in a dough that our updated Kumamax enzyme is inactive in the environment. When the absence of the protein of the gluten our repoter gene is expressed and give red light to environment. Besides LacI promoter works under acidic environment in the dough formation. | ||
| + | <h2 align=center>mCherry(restricted)-GFP-GFP</h2> | ||
| + | https://static.igem.org/mediawiki/2015/thumb/9/97/MCherry%28restricted%29-GFP-GFP-PGap-z.JPG/319px-MCherry%28restricted%29-GFP-GFP-PGap-z.JPG | ||
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| + | <h2 align=center>101-106-117-mcherry-pgap-pgap-pgap-pgap-mcherry</h2> | ||
| + | https://static.igem.org/mediawiki/2015/f/fa/101-106-117-mcherry-pgap-pgap-pgap-pgap-mcherryLAST.PNG | ||
| + | |||
| + | <h2 align=center>KumamolisinMAX-strain3b-KumamolisinMAX-stain4a-2(compability)-mCherry(nice)-1(compability)-mCherry-mCherry</h2> | ||
| + | https://static.igem.org/mediawiki/2015/0/06/Kumma123metu.PNG | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
Latest revision as of 00:11, 12 September 2015
Kumamax enzyme that gluten updated m-cherry reporter device
we modernized m-cherry (BBa_J06504) part to report the absence of the gluten protein in a solution or in a dough that our updated Kumamax enzyme is inactive in the environment. When the absence of the protein of the gluten our repoter gene is expressed and give red light to environment. Besides LacI promoter works under acidic environment in the dough formation.
mCherry(restricted)-GFP-GFP
101-106-117-mcherry-pgap-pgap-pgap-pgap-mcherry
KumamolisinMAX-strain3b-KumamolisinMAX-stain4a-2(compability)-mCherry(nice)-1(compability)-mCherry-mCherry
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 793
Illegal AgeI site found at 905 - 1000COMPATIBLE WITH RFC[1000]