Difference between revisions of "Part:BBa K1755001"
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[[File:LZU_iGEM_2015_ribB_picture.JPG|400px|thumb|left|Normal E.coli(left one)and E.coli with ribB(right one)]] | [[File:LZU_iGEM_2015_ribB_picture.JPG|400px|thumb|left|Normal E.coli(left one)and E.coli with ribB(right one)]] | ||
Revision as of 06:40, 27 August 2015
ribB coding sequence
ribB，the 3,4 dihydroxy-2-butanone-4-phosphate synthase。 We get this part from E.coli genome by PCR. It can increase the electric output by synthesis of riboflavin.
Besides, riboflavin itself is yellow. After the addition of ribB, the bacteria will be yellow.
Usage and Biology
The protein RibB catalyzes the conversion of D-ribulose 5-phosphate to formate and 3,4-dihydroxy-2-butanone 4-phosphate.
This protein is involved in one step of the subpathway that synthesizes 2-hydroxy-3-oxobutyl phosphate from D-ribulose 5-phosphate.
Proteins known to be involved in this subpathway in this organism are: 3,4-dihydroxy-2-butanone 4-phosphate synthase (ribB)
This subpathway is part of the pathway riboflavin biosynthesis, which is itself part of Cofactor biosynthesis.
Catalytic activity
D-ribulose 5-phosphate = formate + L-3,4-dihydroxybutan-2-one 4-phosphate.
Sequence
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 387
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 277
Illegal AgeI site found at 319 - 1000COMPATIBLE WITH RFC[1000]