Difference between revisions of "Part:BBa K1677369:Design"
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It is extracted from the Mouse mammary tumor virus. | It is extracted from the Mouse mammary tumor virus. | ||
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Staple, D. W., Butcher, S. E., Pseudoknots: RNA structures with diverse functions. PLoS biology 2005, 3, e213. <br> | Staple, D. W., Butcher, S. E., Pseudoknots: RNA structures with diverse functions. PLoS biology 2005, 3, e213. <br> | ||
Sperschneider, J., Datta, A., DotKnot: pseudoknot prediction using the probability dot plot under a refined energy model. Nucleic acids research 2010, 38, e103. <br> | Sperschneider, J., Datta, A., DotKnot: pseudoknot prediction using the probability dot plot under a refined energy model. Nucleic acids research 2010, 38, e103. <br> | ||
Revision as of 07:21, 25 August 2015
MMTV Pseudoknot
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
All start codons had to be removed from the pseudoknot.
20 base pairs from the viral DNA flank either side of the pseudoknot region.
3' flanking sequence AAATTCAAAAAACTTGTAAA
5' flanking sequence AAAGGTAAGGACTCAGGATT
The actual pseudoknot is:
GGGGCAGUCCCCUAGCCCCACUCAAAAGGGGGAU
(((((.[[[[[[[.)))))........]]]]]]]
Estimated free energy: -25.93 kcal/mol
Source
It is extracted from the Mouse mammary tumor virus.
big titty bitches
Staple, D. W., Butcher, S. E., Pseudoknots: RNA structures with diverse functions. PLoS biology 2005, 3, e213.
Sperschneider, J., Datta, A., DotKnot: pseudoknot prediction using the probability dot plot under a refined energy model. Nucleic acids research 2010, 38, e103.