Difference between revisions of "Part:BBa K1677369:Design"

(→‎Design Notes)
(→‎References)
Line 22: Line 22:
  
 
===References===
 
===References===
 +
Staple, D. W., Butcher, S. E., Pseudoknots: RNA structures with diverse functions. PLoS biology 2005, 3, e213. <br>

Revision as of 07:18, 25 August 2015


MMTV Pseudoknot


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

All start codons had to be removed from the pseudoknot. 20 base pairs from the viral DNA flank either side of the pseudoknot region. 3' flanking sequence AAATTCAAAAAACTTGTAAA 5' flanking sequence AAAGGTAAGGACTCAGGATT

The actual pseudoknot is: GGGGCAGUCCCCUAGCCCCACUCAAAAGGGGGAU (((((.[[[[[[[.)))))........]]]]]]] Estimated free energy: -25.93 kcal/mol

Source

It is extracted from the Mouse mammary tumor virus.

References

Staple, D. W., Butcher, S. E., Pseudoknots: RNA structures with diverse functions. PLoS biology 2005, 3, e213.