Difference between revisions of "Part:BBa K1758300:Design"

 
 
Line 1: Line 1:
−
 
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K1758300 short</partinfo>
 
<partinfo>BBa_K1758300 short</partinfo>
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Fragment 1:
 
Fragment 1:
 +
 
UTR_arsO_rev: ccttcttttttttttttaaagttaaacaaaattatTAAGTCAAAAACATATATGACTTAACG
 
UTR_arsO_rev: ccttcttttttttttttaaagttaaacaaaattatTAAGTCAAAAACATATATGACTTAACG
 +
 
Cm_fwd: CGGCATCAGCACCTTGTC
 
Cm_fwd: CGGCATCAGCACCTTGTC
 +
 
Template: pSB1C3-pT7-arsR
 
Template: pSB1C3-pT7-arsR
  
 
Fragment 2:
 
Fragment 2:
 +
 
UTR_fwd: GTTTAACTTTAAAAAAAAAAAAGAAGGAGATATACATATGCGTAAAGGCGAAGAGCTGTTCAC  
 
UTR_fwd: GTTTAACTTTAAAAAAAAAAAAGAAGGAGATATACATATGCGTAAAGGCGAAGAGCTGTTCAC  
 +
 
Cm_rev: TATACGCAAGGCGACAAG
 
Cm_rev: TATACGCAAGGCGACAAG
 +
 
Template: BBa_I746909
 
Template: BBa_I746909
  

Latest revision as of 21:37, 22 August 2015

sfGFP controlled by T7 promoter and arsenic operator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 162


Design Notes

This part was created by inserting the T7 promoter BBa_I719005 upstream of BBa_J33201 via 3A assembly. Subsequently, Gibson assembly was performed to replace arsR by sfGFP and introduce the UTR. The followoing primers and templates were used:

Fragment 1:

UTR_arsO_rev: ccttcttttttttttttaaagttaaacaaaattatTAAGTCAAAAACATATATGACTTAACG

Cm_fwd: CGGCATCAGCACCTTGTC

Template: pSB1C3-pT7-arsR

Fragment 2:

UTR_fwd: GTTTAACTTTAAAAAAAAAAAAGAAGGAGATATACATATGCGTAAAGGCGAAGAGCTGTTCAC

Cm_rev: TATACGCAAGGCGACAAG

Template: BBa_I746909


Source

Based on three parts from the distribution.

References