Difference between revisions of "Part:BBa K1758100"

Revision as of 00:23, 22 August 2015

Translation enhancing 5-UTR containing g10-L RBS

This sequence contains a 5' untranslated region (5'-UTR) and a strong ribosomal binding site from bacteriophage T7, named g10-L. This sequence greatly enhances translation of a following gene. The enhancing effect relies on the regulation of mRNA binding to and release of the ribosome S30 subunit (for details see [http://www.ncbi.nlm.nih.gov/pubmed/2676996 Olins et al. 1989] and [http://www.ncbi.nlm.nih.gov/pubmed/23927491 Takahashi et al. 2013]).

g10-L is a strong RBS in E. coli but also well suited for foreign gene expression ([http://www.nature.com/nbt/journal/v9/n5/abs/nbt0591-477.html Rangwala et al. 1991]).

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K1758100 short</partinfo>
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 This sequence contains a 5' untranslated region (5'-UTR) and a strong ribosomal binding site from bacteriophage T7, named ''g10''-L.  This sequence greatly enhances translation of a following gene. The enhancing effect relies on the regulation of mRNA binding to and release of the ribosome S30 subunit (for details see [http://www.ncbi.nlm.nih.gov/pubmed/2676996 Olins et al. 1989] and [http://www.ncbi.nlm.nih.gov/pubmed/23927491 Takahashi et al. 2013]).
-''g10''-L is a strong RBS in ''E. coli'' but also well suited for foreign gene expression ([http://www.nature.com/nbt/journal/v9/n5/abs/nbt0591-477.html Rangwala et al. 1991])
+''g10''-L is a strong RBS in ''E. coli'' but also well suited for foreign gene expression ([http://www.nature.com/nbt/journal/v9/n5/abs/nbt0591-477.html Rangwala et al. 1991]).
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