Difference between revisions of "Part:BBa K1604020:Design"
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===Source=== | ===Source=== | ||
Arabinose inducible araC-pBAD promoter come from E. coli genome. | Arabinose inducible araC-pBAD promoter come from E. coli genome. | ||
| − | Gene coding sequences come from Pantoea ananatis (formerly Erwinia uredovora). | + | Gene coding sequences come from Pantoea ananatis (formerly Erwinia uredovora) genome. |
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===References=== | ===References=== | ||
Revision as of 17:54, 18 August 2015
araC-pBAD + beta-carotene
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1144
Illegal BamHI site found at 3185 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 2721
Illegal NgoMIV site found at 2851
Illegal AgeI site found at 979
Illegal AgeI site found at 1936 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 961
Design Notes
Standard assembly between BBA_K731201 and a PCR amplification of BBa_K274200. BBa_K274200 was extracted by the 2012 distribution kit in pSB1A2 and it was amplified by PCR to restore a missing SpEI site.
Source
Arabinose inducible araC-pBAD promoter come from E. coli genome. Gene coding sequences come from Pantoea ananatis (formerly Erwinia uredovora) genome.