Difference between revisions of "Part:BBa K1604030:Design"
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PncB encodes for the enzyme NAPRTase (nicotinic acid phosphori- bosyltransferase).
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It catalyzes the formation of nicotinate mono-nucleotide, a direct precursor of NAD, from NA.
 
<partinfo>BBa_K1604030 short</partinfo>
 
<partinfo>BBa_K1604030 short</partinfo>
  

Revision as of 17:06, 18 August 2015

PncB encodes for the enzyme NAPRTase (nicotinic acid phosphori- bosyltransferase). It catalyzes the formation of nicotinate mono-nucleotide, a direct precursor of NAD, from NA. pncB


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1106
  • 1000
    COMPATIBLE WITH RFC[1000]

PncB encodes for the enzyme NAPRTase (nicotinic acid phosphori- bosyltransferase). It catalyzes the formation of nicotinate mono-nucleotide, a direct precursor of NAD, from NA.


Design Notes

pst1 illegal site has been removed from the sequence


Source

Escherichia Coli Bl21 (DE3)

References

Susana J. Berrıos-Rivera, K.-Y. San, and G. N. Bennett. (2002) Metabolic Engineering 4, 238–247

Marcel G. Wubbolts, Peter Terpstra, Jan B. van Beilen, Jaap Kingma, H. A. Rene Meesters, and Bernard Witholt. (1990) JBC, 265 (29), 17665-72

Ka-Yiu San, George N. Bennett, Susana J. Berríos-Rivera, Ravi V. Vadali, Yea-Tyng Yang, Emily Horton, Fred B. Rudolph, Berna Sariyar, and Kimathi Blackwood (2002) Metabolic Engineering, 4, 182–192