Difference between revisions of "Part:BBa K1699004"
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<partinfo>BBa_K1699004 short</partinfo>
 
<partinfo>BBa_K1699004 short</partinfo>
  
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gRNA for SaCas9 targeting ubiquitin B under U6 promoter
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gRNA for SaCas9 targeting ubiquitin B under U6 promoter. This gRNA sequence complements 3 different loci in the second exon of human UBB gene, and is under U6 (RNA pol III) promoter.
  
  

Revision as of 08:33, 3 September 2015

gRNA for SaCas9 targeting human ubiquitin B gene under U6 promoter

gRNA for SaCas9 targeting ubiquitin B under U6 promoter. This gRNA sequence complements 3 different loci in the second exon of human UBB gene, and is under U6 (RNA pol III) promoter.


Usage and Biology

usage and biology


Characterization

characterization

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 250
  • 1000
    COMPATIBLE WITH RFC[1000]