Difference between revisions of "Part:BBa K1758101"
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This part includes a translation enhancing sequence, 5'-UTR, in front of sfGFP coding sequence ([https://parts.igem.org/wiki/index.php?title=Part:BBa_I746916 BBa_I746916]). It also contains a double terminator made of [https://parts.igem.org/wiki/index.php?title=Part:BBa_B0010 B0010] and [https://parts.igem.org/wiki/index.php?title=Part:BBa_B0012 B0012]. The translation enhancing sequence improves the binding of the mRNA to the ribosome ([http://www.ncbi.nlm.nih.gov/pubmed/2676996 Olins et al. 1989]) and is especially helpful in ''in vitro'' cell free protein synthesis. The sequence contains a spacer of 10 adenine bases which has been shown to further enhance translation efficiency ([http://www.ncbi.nlm.nih.gov/pubmed/23927491 Takahashi et al. 2013]). | This part includes a translation enhancing sequence, 5'-UTR, in front of sfGFP coding sequence ([https://parts.igem.org/wiki/index.php?title=Part:BBa_I746916 BBa_I746916]). It also contains a double terminator made of [https://parts.igem.org/wiki/index.php?title=Part:BBa_B0010 B0010] and [https://parts.igem.org/wiki/index.php?title=Part:BBa_B0012 B0012]. The translation enhancing sequence improves the binding of the mRNA to the ribosome ([http://www.ncbi.nlm.nih.gov/pubmed/2676996 Olins et al. 1989]) and is especially helpful in ''in vitro'' cell free protein synthesis. The sequence contains a spacer of 10 adenine bases which has been shown to further enhance translation efficiency ([http://www.ncbi.nlm.nih.gov/pubmed/23927491 Takahashi et al. 2013]). | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
+ | <html> | ||
+ | <p>By adding a promoter of choice, sfGFP is produced. The high efficiency of our designed 5'-UTR ensures reporter production even when weak promoters are employed.</p> | ||
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+ | <h2> Characterization </h2> | ||
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+ | <p> This part has been used in a variety of experiments, including experiments with BBa_K1758102, BBa_K1758377, BBa_K1758312, BBa_K1758314, BBa_K1758323, BBa_K1758325, BBa_K1758332 and others. For details see the corresponding part or our wiki. </p> | ||
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+ | </html> | ||
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Revision as of 00:07, 19 September 2015
Translation enhancing 5-UTR + sfGFP
This part includes a translation enhancing sequence, 5'-UTR, in front of sfGFP coding sequence (BBa_I746916). It also contains a double terminator made of B0010 and B0012. The translation enhancing sequence improves the binding of the mRNA to the ribosome ([http://www.ncbi.nlm.nih.gov/pubmed/2676996 Olins et al. 1989]) and is especially helpful in in vitro cell free protein synthesis. The sequence contains a spacer of 10 adenine bases which has been shown to further enhance translation efficiency ([http://www.ncbi.nlm.nih.gov/pubmed/23927491 Takahashi et al. 2013]).
Usage and Biology
By adding a promoter of choice, sfGFP is produced. The high efficiency of our designed 5'-UTR ensures reporter production even when weak promoters are employed.
Characterization
This part has been used in a variety of experiments, including experiments with BBa_K1758102, BBa_K1758377, BBa_K1758312, BBa_K1758314, BBa_K1758323, BBa_K1758325, BBa_K1758332 and others. For details see the corresponding part or our wiki.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 59