Difference between revisions of "Part:BBa K1758101"

Revision as of 16:24, 17 August 2015

Translation enhancing 5-UTR + sfGFP

This part includes a translation enhancing sequence, 5'-UTR, in front of sfGFP coding sequence (BBa_I746916). It also contains a double terminator made of B0010 and B0012. The translation enhancing sequence improves the binding of the mRNA to the ribosome ([http://www.ncbi.nlm.nih.gov/pubmed/2676996 Olins et al. 1989]) and is especially helpful in in vitro cell free protein synthesis. The sequence contains a spacer of 10 adenine bases which has been shown to further enhance translation efficiency ([http://www.ncbi.nlm.nih.gov/pubmed/23927491 Takahashi et al. 2013]).

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 59

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 __NOTOC__
 <partinfo>BBa_K1758101 short</partinfo>
-This part includes a translation enhancing sequence, 5'-UTR, in front of sfGFP coding sequence (BBa_I746916). It also contains a double terminator made of <a href"https://parts.igem.org/wiki/index.php?title=Part:BBa_B0010">B0010</a> and <a href"https://parts.igem.org/wiki/index.php?title=Part:BBa_B0012">B0012</a>. The translation enhancing sequence improves the binding of the mRNA to the ribosome (<a href="http://www.ncbi.nlm.nih.gov/pubmed/2676996">Olins et al. 1989</a>) and is especially helpful in <i>in vitro</i> cell free protein synthesis. The sequence contains a spacer of 10 adenine bases which has been shown to further enhance translation efficiency (<a href="http://www.ncbi.nlm.nih.gov/pubmed/23927491">Takahashi et al. 2013</a>).
+This part includes a translation enhancing sequence, 5'-UTR, in front of sfGFP coding sequence ([https://parts.igem.org/wiki/index.php?title=Part:BBa_I746916 BBa_I746916]). It also contains a double terminator made of [https://parts.igem.org/wiki/index.php?title=Part:BBa_B0010 B0010] and [https://parts.igem.org/wiki/index.php?title=Part:BBa_B0012 B0012]. The translation enhancing sequence improves the binding of the mRNA to the ribosome ([http://www.ncbi.nlm.nih.gov/pubmed/2676996 Olins et al. 1989]) and is especially helpful in ''in vitro'' cell free protein synthesis. The sequence contains a spacer of 10 adenine bases which has been shown to further enhance translation efficiency ([http://www.ncbi.nlm.nih.gov/pubmed/23927491 Takahashi et al. 2013]).
 <!-- Add more about the biology of this part here