Difference between revisions of "Part:BBa K1813034"

 
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<partinfo>BBa_K1813034 short</partinfo>
 
<partinfo>BBa_K1813034 short</partinfo>
  
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LacIR AgCPR CYP6CM1
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A construct used to achieve the simultaneous bacterial expression of CYP6CM1vQ (<html><b><a href="https://parts.igem.org/Part:BBa_K1813005">BBa_K1813005</b></a></html>) and the <i> A. gambiae </i> NADPH p450 reductase (<html><b><a href="https://parts.igem.org/Part:BBa_K1813003">BBa_K1813003</b></a></html>) under regulative authority of the LacI repressor. <br>
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===Functional Parameters===
 
===Functional Parameters===
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<partinfo>BBa_K1813034 parameters</partinfo>
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<partinfo>BBa_K1813024 parameters</partinfo>
 
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<h2> Background </h2>
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Repressed by the presence of the LacI regulatory element, this construct does not allow significant expression of either gene without induction by the presence of Isopropyl β-D-1-thiogalactopyranoside in a bacterial host. In doing so, the cytochrome p450 enzyme receives a steady supply of electrons from the reductase, critical for p450 mediated catalysis, once the construct is expressed.

Revision as of 08:37, 18 September 2015

lacIR, AgCPR and CYP6CM1 Regulon

A construct used to achieve the simultaneous bacterial expression of CYP6CM1vQ (BBa_K1813005) and the A. gambiae NADPH p450 reductase (BBa_K1813003) under regulative authority of the LacI repressor.



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 105
    Illegal BamHI site found at 1887
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1685
    Illegal AgeI site found at 2867
    Illegal AgeI site found at 3023
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 3383


Background

Repressed by the presence of the LacI regulatory element, this construct does not allow significant expression of either gene without induction by the presence of Isopropyl β-D-1-thiogalactopyranoside in a bacterial host. In doing so, the cytochrome p450 enzyme receives a steady supply of electrons from the reductase, critical for p450 mediated catalysis, once the construct is expressed.