Difference between revisions of "Part:BBa K1813032"

 
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<partinfo>BBa_K1813032 short</partinfo>
 
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LacIR AgCPR HUMCYPDB1
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A construct used to achieve the bacterial expression of HUMCYPDB1 (<html><b><a href="https://parts.igem.org/Part:BBa_K1813005">BBa_K1813005</b></a></html> and the <i> A. gambiae </i> NADPH p450 reductase simultaneously (<html><b><a href="https://parts.igem.org/Part:BBa_K1813005">BBa_K1813005</b></a></html>) under regulative authority of the LacI repressor. <br>
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K1813032 SequenceAndFeatures</partinfo>
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<partinfo>BBa_K1813024 SequenceAndFeatures</partinfo>
  
  
 
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===Functional Parameters===
 
===Functional Parameters===
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<partinfo>BBa_K1813032 parameters</partinfo>
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<partinfo>BBa_K1813024 parameters</partinfo>
 
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<h2> Background </h2>
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Repressed by the presence of the LacI regulatory element, this construct does not allow significant expression of either gene without induction by the presence of Isopropyl β-D-1-thiogalactopyranoside in a bacterial host. In doing so, the cytochrome p450 enzyme receives a steady supply of electrons from the reductase, critical for p450 mediated catalysis, once the construct is expressed.

Revision as of 08:27, 18 September 2015

LacIR, AgCPR and CYP2D6 Regulon

A construct used to achieve the bacterial expression of HUMCYPDB1 (BBa_K1813005 and the A. gambiae NADPH p450 reductase simultaneously (BBa_K1813005) under regulative authority of the LacI repressor.



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 105
    Illegal BglII site found at 1737
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2327
  • 1000
    COMPATIBLE WITH RFC[1000]


Background

Repressed by the presence of the LacI regulatory element, this construct does not allow significant expression of either gene without induction by the presence of Isopropyl β-D-1-thiogalactopyranoside in a bacterial host. In doing so, the cytochrome p450 enzyme receives a steady supply of electrons from the reductase, critical for p450 mediated catalysis, once the construct is expressed.