Difference between revisions of "Part:BBa K1783003:Design"

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===Design Notes===
 
===Design Notes===
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Construct was assembled using Gibson assembly of K1783001 (Hok-Sok) and K1783002 (Const. Unstable RFP). PCR of Hok-Sok component was difficult due to high inherent secondary structure of the gene sequences. DMSO was needed in order to break this structure.
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Construct was assembled using Gibson assembly of a G-block of K1783001 (Hok/Sok) and K1783002 (Const. Unstable RFP) into a PSB1C3 backbone.  
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PCR for primer extension of Hok/Sok component was difficult due to high inherent secondary structure of the gene sequences. Therefore, the Gibson Assembly of the Hok/Sok and Const. Unstable RFP proved to be difficult.  
  
 
===Source===
 
===Source===

Revision as of 03:55, 26 September 2015

Unstable RFP under Hok/Sok Regulation


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 587
    Illegal NheI site found at 610
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1199
    Illegal AgeI site found at 1311
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Construct was assembled using Gibson assembly of a G-block of K1783001 (Hok/Sok) and K1783002 (Const. Unstable RFP) into a PSB1C3 backbone. PCR for primer extension of Hok/Sok component was difficult due to high inherent secondary structure of the gene sequences. Therefore, the Gibson Assembly of the Hok/Sok and Const. Unstable RFP proved to be difficult.

Source

See construction parts.

References