Difference between revisions of "Part:BBa K1638014"
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Peptide aptamers are combinatorial recognition proteins that provide high specificity and strong binding affinity. They consist of a variable peptide sequence inserted into a protein scaffold. This variable peptide loop makes up a binding domain that enables binding to various proteins. | Peptide aptamers are combinatorial recognition proteins that provide high specificity and strong binding affinity. They consist of a variable peptide sequence inserted into a protein scaffold. This variable peptide loop makes up a binding domain that enables binding to various proteins. | ||
| − | The design of this peptide aptamer scaffold is inspired by (Borghouts C et al., 2008) [1]. | + | The design of this peptide aptamer scaffold is inspired by (Borghouts C et al., 2008) [1]. The scaffold is based on the human thioredoxin that has been modified by the replacement of five cysteins with two glycins and three serines. This includes two cysteins in the active site: |
| + | |||
| + | - C23>G | ||
Revision as of 13:12, 14 August 2015
hTrx scaffold for peptide aptamers with 3xFLAG-tag
This part is a human thioredoxin-based scaffold for the presentation of peptide aptamers. The hTrx-scaffold contains a xhoI restriction site that enables insertion of a random DNA library. A 3xFLAG-tag is added to the C-terminal end of the scaffold. This affinity tag can be used for detection and/or purification purposes.
An antibody-mimetic
Peptide aptamers are combinatorial recognition proteins that provide high specificity and strong binding affinity. They consist of a variable peptide sequence inserted into a protein scaffold. This variable peptide loop makes up a binding domain that enables binding to various proteins.
The design of this peptide aptamer scaffold is inspired by (Borghouts C et al., 2008) [1]. The scaffold is based on the human thioredoxin that has been modified by the replacement of five cysteins with two glycins and three serines. This includes two cysteins in the active site:
- C23>G
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 100
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
References
[1] Borghouts C, Kunz C, Delis N, Groner B. Monomeric Recombinant Peptide Aptamers Are Required for Efficient Intracellular Uptake and Target Inhibition. Molecular Cancer Research. 2008;6(2):267-81.