Difference between revisions of "Part:BBa K1638014"
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| − | + | This part is a human thioredoxin-based scaffold for the presentation of peptide aptamers. The hTrx-scaffold contains a xhoI restriction site that enables insertion of a random DNA library. A 3xFLAG-tag is added to the C-terminal end of the scaffold. This affinity tag can be used for detection and/or purification purposes. | |
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| + | ===An antibody-mimetic=== | ||
| + | Peptide aptamers are combinatorial recognition proteins that provide high specificity and strong binding affinity. They consist of a variable peptide sequence inserted into a protein scaffold. This variable peptide loop makes up a binding domain that enables binding to various proteins. | ||
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| + | The design of this peptide aptamer scaffold is inspired by (Borghouts C et al., 2008) [1]. | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
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Revision as of 12:58, 14 August 2015
hTrx scaffold for peptide aptamers with 3xFLAG-tag
This part is a human thioredoxin-based scaffold for the presentation of peptide aptamers. The hTrx-scaffold contains a xhoI restriction site that enables insertion of a random DNA library. A 3xFLAG-tag is added to the C-terminal end of the scaffold. This affinity tag can be used for detection and/or purification purposes.
An antibody-mimetic
Peptide aptamers are combinatorial recognition proteins that provide high specificity and strong binding affinity. They consist of a variable peptide sequence inserted into a protein scaffold. This variable peptide loop makes up a binding domain that enables binding to various proteins.
The design of this peptide aptamer scaffold is inspired by (Borghouts C et al., 2008) [1].
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 100
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
References
[1] Borghouts C, Kunz C, Delis N, Groner B. Monomeric Recombinant Peptide Aptamers Are Required for Efficient Intracellular Uptake and Target Inhibition. Molecular Cancer Research. 2008;6(2):267-81.