Difference between revisions of "Part:BBa K1632003"
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<partinfo>BBa_K1632003 short</partinfo> | <partinfo>BBa_K1632003 short</partinfo> | ||
| − | + | The <i>fim</i> switch is the promoter containing repeated DNA sequence which is inverted by the Fim recombinase. Therefore, we can control the expression of the gene downstream of the <i>fim</i> switch by adding the Fim recombinase.<br> | |
| + | This fim switch has a J23119 promoter. Also between the promoter and the inverting site, there are restriction enzyme sites. Therefore, the promoter can easily be interchanged. | ||
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Revision as of 09:03, 12 September 2015
fim switch[default OFF](Tokyo_Tech/J23119)_rbs_gfp
The fim switch is the promoter containing repeated DNA sequence which is inverted by the Fim recombinase. Therefore, we can control the expression of the gene downstream of the fim switch by adding the Fim recombinase.
This fim switch has a J23119 promoter. Also between the promoter and the inverting site, there are restriction enzyme sites. Therefore, the promoter can easily be interchanged.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 98
Illegal NheI site found at 121 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 92
Illegal BamHI site found at 133 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1102