Difference between revisions of "Part:BBa K1699002:Design"

 
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<partinfo>BBa_K1699002 short</partinfo>
 
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===References===
 
===References===
 +
 +
1. Self-processing of ribozyme-flanked RNAs into guide RNAs in vitro and in vivo for CRISPR-mediated genome editing
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http://onlinelibrary.wiley.com/doi/10.1111/jipb.12152/full

Revision as of 08:29, 3 September 2015

gRNA for SaCas9 targeting human ubiquitin B gene


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 146
    Illegal NgoMIV site found at 175
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

design


Source

source

References

1. Self-processing of ribozyme-flanked RNAs into guide RNAs in vitro and in vivo for CRISPR-mediated genome editing http://onlinelibrary.wiley.com/doi/10.1111/jipb.12152/full