Difference between revisions of "Part:BBa K1582004:Design"
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Revision as of 09:06, 7 August 2015
[[File:Media:Example.jpg]] GFP+sJanus Fusion Protein
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 976
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 644
Illegal SapI site found at 985
Design Notes
When amplify the HFBI and GFP with PCR, temperature is important.
Source
GFP comes frome iGEM's standard part and HFBI comes frome Trichoderma reesei.