Difference between revisions of "Part:BBa K1582004"

Revision as of 09:05, 7 August 2015

GFP+sJanus Fusion Protein

HFBI is a modle of class I of hydrophobins, which are naturally produced by filamentous fungi. Hydrophobins are amphipathic, which means they have the hydrophobic part and hydrophilic part at the same time. HFBI is a kind of hydrophobins and it has magic properties. We combine GFP with HFBI by a linker and make them become a fusion protein. This fusion protein could be used in aqueous two-phase systems to make itself seperated frome mixture. Meanwile, hydrophobin has a property that it can generate a membrane on the surface of solid automatically, which is called self-assembly. We can get a fluorescent protein' membrane by it.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 976
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 644
Illegal SapI site found at 985

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 __NOTOC__
 <partinfo>BBa_K1582004 short</partinfo>
-HFBI is a modle of class I of hydrophobins, which are naturally produced by filamentous fungi. Hydrophobins are amphipathic, which means they have the hydrophobic part and hydropholic part at the same time. HFBI is a kind of hydrophobins and it has magic properties. We combine GFP with HFBI by a linker and make them become a fusion protein. This fusion protein could be used in aqueous two-phase systems to make itself seperated frome mixture. Meanwile, hydrophobin has a property that it can generate a membrane on the surface of solid automatically, which is called self-assembly. We can get a fluorescent protein' membrane by it.
+HFBI is a modle of class I of hydrophobins, which are naturally produced by filamentous fungi. Hydrophobins are amphipathic, which means they have the hydrophobic part and hydrophilic part at the same time. HFBI is a kind of hydrophobins and it has magic properties. We combine GFP with HFBI by a linker and make them become a fusion protein. This fusion protein could be used in aqueous two-phase systems to make itself seperated frome mixture. Meanwile, hydrophobin has a property that it can generate a membrane on the surface of solid automatically, which is called self-assembly. We can get a fluorescent protein' membrane by it.
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