Difference between revisions of "Part:BBa K1813012"

 
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<partinfo>BBa_K1813012 short</partinfo>
 
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A construct used to achieve the overexpression of CYP6G1(( <html><b><a href="https://parts.igem.org/Part:BBa_K1813004">BBa_K181304</b></a></html>)
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ptac CYP6G1 term
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Structure: ptac:: CYP6G1:: terminator.
  
 
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<partinfo>BBa_K1813012 parameters</partinfo>
 
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<h2> Background </h2>
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Powered by the constitutive bacterial promoter ptac, this construct allows the indiscriminate production of CYP6G1 by a bacterium harbouring this construct.
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This construct was assembled by Michael VanInsberghe, and is codon optimized for <i> E. coli </i>. <br>
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It is used in <html><b><a href="https://parts.igem.org/Part:BBa_K1813025">BBa_K1813025</b></a></html> and <html><b><a href="https://parts.igem.org/Part:BBa_K1813033">BBa_K1813033</b></a></html>.

Revision as of 06:32, 18 September 2015

CYP6G1 Expression Cassette A construct used to achieve the overexpression of CYP6G1(( BBa_K181304) Structure: ptac:: CYP6G1:: terminator.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1021
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1030


Background

Powered by the constitutive bacterial promoter ptac, this construct allows the indiscriminate production of CYP6G1 by a bacterium harbouring this construct. This construct was assembled by Michael VanInsberghe, and is codon optimized for E. coli .
It is used in BBa_K1813025 and BBa_K1813033.