Difference between revisions of "Part:BBa K1638007"

Revision as of 10:56, 18 September 2015

GFP reporter with flexible linker

This part codes for a green fluorescent protein (GFP) reporter with a flexible 10 aa linker at N-terminus. Intended for use in protein-GFP fusions, where this part can be suffixed to various proteins. The protein-GFP fusion can be used as a reporter of gene expression and used in localization assays. A TEV-protease recognition site enables separation of the two proteins. This can be useful in different occasions, e.g. protein purification. This part also contains two transcriptional terminators.

Fluorescence wavelengths

Excitation max - 501nm
Emission max - 511nm

For further description of the GFP read here.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 1
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 674

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K1638007 short</partinfo>
-This part codes for a GFP reporter with a flexible 10 aa linker at N-terminus. Intended for use in protein-GFP fusions, where this part can be suffixed to various proteins. The protein-GFP fusion can be used as a reporter of gene expression.
+This part codes for a green fluorescent protein (GFP) reporter with a flexible 10 aa linker at N-terminus. Intended for use in protein-GFP fusions, where this part can be suffixed to various proteins. The protein-GFP fusion can be used as a reporter of gene expression and used in localization assays. A TEV-protease recognition site enables separation of the two proteins. This can be useful in different occasions, e.g. protein purification.
 This part also contains two transcriptional terminators.
 ====Fluorescence wavelengths====