Difference between revisions of "Part:BBa K1598002:Experience"

 
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===Applications of BBa_K1598002===
 
===Applications of BBa_K1598002===
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==Squirrel-CHN-II 2023==
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To better understand the TPH activity in the intestine, we decided to use a hybrid of in silico and in vivo experiments to investigate the TPH activity. The tryptophan hydroxylases (TPH) from human, as well as from mice, zebrafish, and rabbits have been selected and analyzed for their catalytic efficiency in the rate-limiting step of producing serotonin. By predicting the binding affinity of the TPHs to the tryptophan molecule we could predict the catalytic effectiveness of the enzyme selected.
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In silico binding affinity prediction:
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The full-length 3D structures of the four TPHs from human, mouse, zebrafish, and rabbit are acquired as the PDB files from the uniport database under the following entry numbers: P17752; P09810; Q6PBX4; P17290 for each host organism respectively. For entry numbers P09810, Q6PBX4, and P17290, the known x-ray/NMR structures of the proteins are missing. Instead, the Alphafold predicted full-length structure of the proteins was selected for further analysis.
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<hmtl><img src="https://static.igem.wiki/teams/5009/wiki/basic-parts/homo-tph/homo-tph-1.png" width="80%"></html>
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<hmtl><img src="https://static.igem.wiki/teams/5009/wiki/basic-parts/homo-tph/homo-tph-2.png" width="80%"></html>
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<hmtl><img src="https://static.igem.wiki/teams/5009/wiki/basic-parts/homo-tph/homo-tph-3.png" width="80%"></html>
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<hmtl><img src="https://static.igem.wiki/teams/5009/wiki/basic-parts/homo-tph/homo-tph-4.png" width="80%"></html>
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<hmtl><img src="https://static.igem.wiki/teams/5009/wiki/basic-parts/homo-tph/homo-tph-5.png" width="80%"></html>
  
 
===User Reviews===
 
===User Reviews===

Revision as of 01:00, 12 October 2023


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K1598002

Squirrel-CHN-II 2023

To better understand the TPH activity in the intestine, we decided to use a hybrid of in silico and in vivo experiments to investigate the TPH activity. The tryptophan hydroxylases (TPH) from human, as well as from mice, zebrafish, and rabbits have been selected and analyzed for their catalytic efficiency in the rate-limiting step of producing serotonin. By predicting the binding affinity of the TPHs to the tryptophan molecule we could predict the catalytic effectiveness of the enzyme selected.

In silico binding affinity prediction: The full-length 3D structures of the four TPHs from human, mouse, zebrafish, and rabbit are acquired as the PDB files from the uniport database under the following entry numbers: P17752; P09810; Q6PBX4; P17290 for each host organism respectively. For entry numbers P09810, Q6PBX4, and P17290, the known x-ray/NMR structures of the proteins are missing. Instead, the Alphafold predicted full-length structure of the proteins was selected for further analysis.

<hmtl><img src="homo-tph-1.png" width="80%"></html>

<hmtl><img src="homo-tph-2.png" width="80%"></html>

<hmtl><img src="homo-tph-3.png" width="80%"></html>

<hmtl><img src="homo-tph-4.png" width="80%"></html>

<hmtl><img src="homo-tph-5.png" width="80%"></html>

User Reviews

UNIQ646cf370395eaa9a-partinfo-00000000-QINU UNIQ646cf370395eaa9a-partinfo-00000001-QINU