Difference between revisions of "Part:BBa K1648000"
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[[File:K1648000_Map.png|350px|thumb|right|Construct Map for K1648000]]
 
[[File:K1648000_Map.png|350px|thumb|right|Construct Map for K1648000]]
  
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Consist of four fragments from magnetosome island of ''Magnetospirillum gryphiswaldense'' genome.
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Consists of four fragments from magnetosome island of ''Magnetospirillum gryphiswaldense'' genome.
  
 
Due to potential difficulty in introducing the large magnetosome formation operons into ''Azotobacter vinelandii'', a "template" consists of flanking sequences from '''mamAB operon''' is designed for '''homologous recombination'''.
 
Due to potential difficulty in introducing the large magnetosome formation operons into ''Azotobacter vinelandii'', a "template" consists of flanking sequences from '''mamAB operon''' is designed for '''homologous recombination'''.
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Part containing this template ([https://parts.igem.org/Part:BBa_K1648xxx K1648XXX]) is introduced into ''A. vinelandii'' genome via '''random integration''' before full operon sequences to be transformed into ''A. vinelandii''.
 
Part containing this template ([https://parts.igem.org/Part:BBa_K1648xxx K1648XXX]) is introduced into ''A. vinelandii'' genome via '''random integration''' before full operon sequences to be transformed into ''A. vinelandii''.
  
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===

Revision as of 16:30, 15 July 2015

Recombination Template for mamAB Operon

Construct Map for K1648000

Consists of four fragments from magnetosome island of Magnetospirillum gryphiswaldense genome.

Due to potential difficulty in introducing the large magnetosome formation operons into Azotobacter vinelandii, a "template" consists of flanking sequences from mamAB operon is designed for homologous recombination.

Part containing this template (K1648XXX) is introduced into A. vinelandii genome via random integration before full operon sequences to be transformed into A. vinelandii.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 775
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 291
    Illegal AgeI site found at 810
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 536