Difference between revisions of "Part:BBa K1648000"

Revision as of 01:56, 15 July 2015

Recombination Template for mamAB Operon

Construct Map for K1648000

Consist of four fragments from magnetosome island of Magnetospirillum gryphiswaldense genome.

Due to potential difficulty in introducing the large magnetosome formation operons into Azotobacter vinelandii, a "template" consists of flanking sequences from mamAB operon is designed for homologous recombination.

Part containing this template (K1648XXX) is introduced into A. vinelandii genome via random integration before full operon sequences to be transformed into A. vinelandii.

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 775
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 291
Illegal AgeI site found at 810
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 536

@@ Line 5: / Line 5: @@
 Consist of four fragments from magnetosome island of ''Magnetospirillum gryphiswaldense'' genome.
-Due to potential difficulty in introducing the large magnetosome formation operons into ''Azotobacter vinelandii'', a "template" consists of flanking sequences from '''mamAB operon''' is designed for homologous recombination before full operon sequences to be transformed into ''A. vinelandii''.
+Due to potential difficulty in introducing the large magnetosome formation operons into ''Azotobacter vinelandii'', a "template" consists of flanking sequences from '''mamAB operon''' is designed for '''homologous recombination'''.
+Part containing this template ([https://parts.igem.org/Part:BBa_K1648xxx K1648XXX]) is introduced into ''A. vinelandii'' genome via '''random integration''' before full operon sequences to be transformed into ''A. vinelandii''.
 <!-- Add more about the biology of this part here