Difference between revisions of "Part:BBa K1648000"

Revision as of 16:59, 14 July 2015

Recombination Template for mamAB Operon

Construct Map for K1648000

Consist of four fragments from magnetosome island of Magnetospirillum gryphiswaldense genome.

Due to potential difficulty in introducing the large magnetosome formation operons into Azotobacter vinelandii, a "template" consists of flanking sequences from mamAB operon is designed for homologous recombination before full operon sequences to be transformed into A. vinelandii.

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 775
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 291
Illegal AgeI site found at 810
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 536

@@ Line 3: / Line 3: @@
 [[File:K1648000_Map.png|350px|thumb|right|Construct Map for K1648000]]
-This part is constructed by four fragments from the magnetospirillum gryphiswaldense magnetosome island, and it is to be used to do the homologous recombination with the genomic DNA of the magnetospirillum to form the whole coding sequence of mamAB(a part of genome from magnetospirillum gryphiswaldense magnetosome island).
+Consist of four fragments from magnetosome island of ''Magnetospirillum gryphiswaldense'' genome.
+Due to potential difficulty in introducing the large magnetosome formation operons into ''Azotobacter vinelandii'', a "template" consists of flanking sequences from '''mamAB operon''' is designed for homologous recombination before full operon sequences to be transformed into ''A. vinelandii''.
 <!-- Add more about the biology of this part here