Difference between revisions of "Part:BBa K1648000"
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[[File:K1648000_Map.png|350px|thumb|right|Construct Map for K1648000]] | [[File:K1648000_Map.png|350px|thumb|right|Construct Map for K1648000]] | ||
| − | This part is constructed by four fragments from the magnetospirillum gryphiswaldense magnetosome island, and it is to be used to do the homologous recombination with the genomic DNA of the magnetospirillum to form the whole coding sequence of | + | This part is constructed by four fragments from the magnetospirillum gryphiswaldense magnetosome island, and it is to be used to do the homologous recombination with the genomic DNA of the magnetospirillum to form the whole coding sequence of mamAB(a part of genome from magnetospirillum gryphiswaldense magnetosome island). |
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Revision as of 16:05, 14 July 2015
Recombination Template for mamAB Operon
This part is constructed by four fragments from the magnetospirillum gryphiswaldense magnetosome island, and it is to be used to do the homologous recombination with the genomic DNA of the magnetospirillum to form the whole coding sequence of mamAB(a part of genome from magnetospirillum gryphiswaldense magnetosome island).
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 775
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 291
Illegal AgeI site found at 810 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 536