Difference between revisions of "Part:BBa K1648000:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | In order to form a homologous recombination template, we select 250 bp of each fragment and do the overlapping PCR ligate fragments together. | + | In order to form a homologous recombination template, we select 250 bp of each fragment as the flanking sequence and do the overlapping PCR to ligate fragments together. |
[[File:K1648000 History.png|900px]] | [[File:K1648000 History.png|900px]] | ||
Revision as of 16:11, 14 July 2015
Recombination Template for mamAB Operon
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 775
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 291
Illegal AgeI site found at 810 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 536
Design Notes
In order to form a homologous recombination template, we select 250 bp of each fragment as the flanking sequence and do the overlapping PCR to ligate fragments together.
Source
Magnetospirillum gryphiswaldense MSR-1 v2, complete genome