Difference between revisions of "Part:BBa K1648048:Experience"

 
Line 1: Line 1:
 
 
__NOTOC__
 
__NOTOC__
This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
+
how you used this part and how it worked out.
+
[[File:K1648048_dd.png|350px|thumb|right|Double digestion of K1648048:  L: DNA ladder. Lane 1-3: Recombination Template for J13002-oprF(BBa_K1648048) with double digestion cut at EcoR1 and PstI sites, with single digestion at Pst1 site, without digestion.]]
 +
 
  
 
===Applications of BBa_K1648048===
 
===Applications of BBa_K1648048===
 
+
An ongoing experiment will be carried out for the comparison between the transformed bacteria with [https://parts.igem.org/Part:BBa_K1648048 BBa_K1648048] for oprF from Azotobacter vinelandii [https://parts.igem.org/Part:BBa_K1648049 BBa_K1648049] for mutated oprF from Azotobacter vinelandii and [https://parts.igem.org/Part:BBa_K1648050 BBa_K1648050] for [https://parts.igem.org/Part:BBa_K1172501 BBa_K1172501] from Germany iGEM team. For comparison,an identical promoter, J13002, which is a constitutive promoter, is chosen to add before the gene in psb1C3 for constitutive expression of different OprF in bacteria.
 
===User Reviews===
 
===User Reviews===
 
<!-- DON'T DELETE --><partinfo>BBa_K1648048 StartReviews</partinfo>
 
<!-- DON'T DELETE --><partinfo>BBa_K1648048 StartReviews</partinfo>

Revision as of 04:51, 20 September 2015


Double digestion of K1648048: L: DNA ladder. Lane 1-3: Recombination Template for J13002-oprF(BBa_K1648048) with double digestion cut at EcoR1 and PstI sites, with single digestion at Pst1 site, without digestion.


Applications of BBa_K1648048

An ongoing experiment will be carried out for the comparison between the transformed bacteria with BBa_K1648048 for oprF from Azotobacter vinelandii BBa_K1648049 for mutated oprF from Azotobacter vinelandii and BBa_K1648050 for BBa_K1172501 from Germany iGEM team. For comparison,an identical promoter, J13002, which is a constitutive promoter, is chosen to add before the gene in psb1C3 for constitutive expression of different OprF in bacteria.

User Reviews

UNIQ9d0066eff1290bb7-partinfo-00000000-QINU UNIQ9d0066eff1290bb7-partinfo-00000001-QINU