Difference between revisions of "Part:BBa K1648045:Design"

(Source)
Line 1: Line 1:
 
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K1648045 short</partinfo>
 
<partinfo>BBa_K1648045 short</partinfo>
Line 10: Line 9:
  
 
>SH3 Domain F<br>
 
>SH3 Domain F<br>
GTTTCTTCGAATTCGCGGCCGCTTCTAGAATGGCAGAGTATGTGCGGGC
+
GTTTCTTCGAATTCGCGGCCGCTTCTAGatgaaactgaaaaacaccttgggcg
  
 
>SH3 Domain R<br>
 
>SH3 Domain R<br>
GTTTCTTCCTGCAGCGGCCGCTACTAGTATACTTCTCCACGTAAGGGACAGG
+
GTTTCTTCCTGCAGCGGCCGCTACTAGTAttactgggcttgggcttccac
 
+
 
===Source===
 
===Source===
 
BBa_K1314016
 
BBa_K1314016
  
 
===References===
 
===References===

Revision as of 07:54, 17 September 2015

AvOprF


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 475


Design Notes

PCR with BBa_K1314016 as template using the following primers:

>SH3 Domain F
GTTTCTTCGAATTCGCGGCCGCTTCTAGatgaaactgaaaaacaccttgggcg

>SH3 Domain R
GTTTCTTCCTGCAGCGGCCGCTACTAGTAttactgggcttgggcttccac

Source

BBa_K1314016

References